Enucleation and reconstruction of interferon-producing cells.

P-82: Effect of SCNT Steps on Relative mRNA Abundances of Sheep Oocytes

Background: The oocyte is a unique cell committed to reprogram fertilizing sperm and to support early stages of embryonic development until the species-specific stage of zygote genome activation that occurs around the second to third cell cycle in sheep embryos. In this sense, considering the huge list of oocyte transcripts, we selected some candidates genes based on their roles of regulating di...

Action of interferon in enucleated cells.

Interferon induces protection of enucleated BSC-1 cells against infectious vesicular stomatitis virus production if cells are treated before, but not after, enucleation.

Inducible Expression of Human Gamma Interferon

Background:The premature termination of high producer clones, which will be killed due to cell proliferation and proteins production antagonism, is one of the basic drawback in recombinant proteins technology. Furtheremore, it is supposed some toxic proteins like interferon which we intended to clone and express, inhibit host cells’ proliferation. So, it is necessary to tightly control IFN-γ pr...

Fed-batch Cultivation of Recombinant Escherichia coli Producing Human Interferon-γ Under Controlled Specific Growth Rate

The Effect of Beta Interferon on Dendritic Cells and Cytokine Synthesis by CD4+ T Cells

Background: Dendritic cells (DC) are a key regulator of the immune response, and interferon- beta (IFN-β) is considered an immunomodulatory molecule for DC. Objective: The purpose of this study was to evaluate the ability of IFN-β treated DC to induce cytokine secretion by CD4+ T cells. Methods: Dendritic cells were generated from blood monocytes with granulocyte-monocyte colony-stimulating fac...